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Δευτέρα, 28 Δεκεμβρίου 2009
CD3,CD5,CD19,CD27,CD70 on lymphocytes T and B - Adenoids
The mining of antigen CD27 on lymphocytes T and B in creation of memory cells is still unclear.
CD27 on lymphocytes T and B has a crucial role in development of immune response against inflammatory state. Aim of this study was evaluation functions of lymhocytes with expression CD27 in hypertrophied adenoid in children with otitis media with effusion.
MATHERIAL AND METHODS:
We tested 24 children in examine group with hypertrophied adenoid and otitis media with effusion (OME), and 29 children in comparative group only with hypertrophied adenoid (HA).
We also divided both groups into two groups, above 5 and over 5 years old.
We made the research by flow cytometry method.
We used anty-CD19, anty-CD3, anty-CD5 and anty-CD27 monoclonal antibodies to examinations.
We showed the lowest percentage lymphocytes B CD5+ with expression of CD27 in subgroup older children with otitis media with effusion (O.M.E. 22.43 +/- 2.66%) in compartative to younger children in the same group (15.96 +/- 5.31%; p < .005) and to older comparative subgroup (H.A. 15.50 +/- 8.03; p < .001).
Percentages of B CD5 cells with expression of CD27 was statistical lower in examinated group (7.25 +/- 3.81) than in comparative group (H.A. 16.26 +/- 5.82; p < .04).
In group with hypertrophied adenoid showed higher percentages of CD5-CD27+CD19+ cells both in younger and older supgroup than in equivalent supgroups with O.M.E.
Iteraction of CD27-CD70 regulates many functions of T and B lymphocytes.
It seems that disorders humoral immunological answer in hypertrophied adenoids are supported to develop of otitis media with effusion.
Antigens, CD3 :: metabolism;
Antigens, CD5 :: metabolism;
Antigens,CD19 :: metabolism;
Antigens, CD27 :: metabolism;
B-Lymphocytes :: immunology;
Biological Markers :: metabolism;
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Modern challenged parts of the oath:
- To teach medicine to the sons of my teacher. In the past, medical schools gave preferential consideration to the children of physicians.
- To practice and prescribe to the best of my ability for the good of my patients, and to try to avoid harming them. This beneficial intention is the purpose of the physician. However, this item is still invoked in the modern discussions of euthanasia.
- I will not give a lethal drug to anyone if I am asked, nor will I advise such a plan. Physician organizations in most countries have strongly denounced physician participation in legal executions. However, in a small number of cases, most notably the U.S. states of Oregon, Washington,Montana, and in the Kingdom of the Netherlands, a doctor can prescribe euthanasia with the patient's consent.
- Similarly, I will not give a woman a pessary to cause an abortion. Since the legalization of abortion in many countries, the inclusion of the anti-abortion sentence of the Hippocratic oath has been a source of contention.
- To avoid violating the morals of my community. Many licensing agencies will revoke a physician's license for offending the morals of the community ("moral turpitude").
- I will not cut for stone, even for patients in whom the disease is manifest; I will leave this operation to be performed by practitioners, specialists in this art. The "stones" referred to are kidney stones or bladder stones, removal of which was judged too menial for physicians, and therefore was left for barbers (the forerunners of modern surgeons). Surgery was not recognized as a specialty at that time. This sentence is now interpreted as acknowledging that it is impossible for any single physician to maintain expertise in all areas. It also highlights the different historical origins of the surgeon and the physician.
- To keep the good of the patient as the highest priority. There may be other conflicting 'good purposes,' such as community welfare, conserving economic resources, supporting the criminal justice system, or simply making money for the physician or his employer that provide recurring challenges to physicians